Journal: PLoS Pathogens
Article Title: Therapeutic efficacy of favipiravir against Bourbon virus in mice
doi: 10.1371/journal.ppat.1007790
Figure Lengend Snippet: BRBV-STL was identified in a blood sample from a hospitalized patient. The genome sequence of the virus was determined by next-generation sequencing on RNA extracted from culture supernatant of Vero cells inoculated with the patient serum. ( A ) Phylogenetic tree of segment 2 (PB1 gene) of different orthomyxoviruses. The tree was constructed using ClustalW in the DNASTAR Lasergene 15 software package. Bootstrap values were calculated on a 1000 trails and the values are included in the figure. NA, no value available. Bourbon virus strain St. Louis (BRBV-STL, MK453528); Bourbon virus strain original (BRBV-KS, KU708254.1); Dhori virus (NC_034263.1); Oz virus (LC320124.1); Jos virus (HM627170.1); Aransas virus (KC506163.1); Thogoto virus (AF004985.1); Influenza A virus (CY009450); Influenza B virus (AY582058.1); Influenza C virus (NC_006308.2); Influenza D virus (LN559121.1); Quaranfil virus (FJ861695.1). BRBV-STL belongs to the Thogotovirus genus (grey background) and is very similar to the original strain of BRBV. ( B ) Percent identity at the nucleotide (nt) level between our BRBV isolate (BRBV-STL) and the original BRBV isolate (BRBV-KS).
Article Snippet: A phylogenetic tree of segment 2 (PB1 gene) of different orthomyxoviruses was constructed using ClustalW in the DNASTAR Lasergene 15 software package.
Techniques: Sequencing, Virus, Next-Generation Sequencing, Construct, Software